Bout A was from an untrained individual

Bout A was from an
untrained individual and Bout B was from a trained individual you can see from
the data why this is. After exercise concentration of lactate was lower in Bout
B than A which is typical of a trained individual. Lactate is the product
formed by the incomplete breakdown of glucose from glycogen which allows
continued ATP production under anaerobic conditions. If the intensity is high
and the individual is untrained (low aerobic capacity) the rate at which
Lactate is cleared reduces becoming lower than the rate of production, so there
is an increased blood lactate concentration. As an individual becomes trained
there aerobic fitness increases resulting in an increased buffering process of
lactate from the blood.

Readings were taken every
5 minutes until the absorbance value stopped increasing, starting with the
2.0nM lactate concentration because this would take the longest to reach
completion.  

Best services for writing your paper according to Trustpilot

Premium Partner
From $18.00 per page
4,8 / 5
4,80
Writers Experience
4,80
Delivery
4,90
Support
4,70
Price
Recommended Service
From $13.90 per page
4,6 / 5
4,70
Writers Experience
4,70
Delivery
4,60
Support
4,60
Price
From $20.00 per page
4,5 / 5
4,80
Writers Experience
4,50
Delivery
4,40
Support
4,10
Price
* All Partners were chosen among 50+ writing services by our Customer Satisfaction Team

The 2 sets of data show a
similar pattern of increased absorbance with increased lactate concentration,
this is because there was more NAD. At 0.2nM and 1.2nM sample 2 showed a much
larger absorbance than sample, this could have been due to human error:

–         
Releasing the plunger
to quickly so the right amount of fluid isn’t drawn into the pipette- this
would have altered the concentration of the sample

–         
2nd
click didn’t release the last drop – would alter the concentration of the
sample as it didn’t contain the correct amount of fluid

–         
Didn’t mix the
reagent and lactate well enough – the reaction didn’t take place properly

If you were to carry out
the experiment again with an increased concentration of the enzyme Lactate
Dehydrogenase the reaction would reach completion quicker; this is because enzymes
are biological catalysts lowering the activation energy thus increasing the
rate of reaction.

If you increased the
concentration of NAD+ in the reagent you would see an increase in absorbance
because there will be more NADH being formed as the amount of NADH formed is
directly proportional to the amount of lactate and NAD+ present:

Lactate +NAD+ (Lactate
dehydrogenase) à
pyruvate +NADH + H+